7-Chloro-5-(1H-pyrrol-2-yl)-1H-benzo[e][1,4]diazepin-2(3H)-one
Ro 5-3335
CAS: 30195-30-3
Molecular Formula: C13H10ClN3O
7-Chloro-5-(1H-pyrrol-2-yl)-1H-benzo[e][1,4]diazepin-2(3H)-one - Names and Identifiers
7-Chloro-5-(1H-pyrrol-2-yl)-1H-benzo[e][1,4]diazepin-2(3H)-one - Physico-chemical Properties
| Molecular Formula | C13H10ClN3O |
| Molar Mass | 259.69 |
| Density | 1.46 g/cm3 |
| Melting Point | 256 °C(dec.) |
| Boling Point | 501.6 °C at 760 mmHg |
| Flash Point | 257.1 °C |
| Appearance | Slightly yellowish to reddish yellow crystals or powder |
| Storage Condition | 2-8℃ |
| MDL | MFCD00870704 |
| In vitro study | Ro5-3335 has antiproliferative activity against human CBF leukemia cell lines, with IC 50 s of 1.1 μM, 21.7 μM and 17.3 μM for ME-1, Kasumi-1 and REH, respectively. Ro5-3335 inhibits definitive hematopoiesis in zebrafish embryos. Ro5-3335 does not completely break apart RUNX1-CBFβ interaction, but changes the conformation of their complex or increases the distance between RUNX1 and CBFβ in the complex. |
| In vivo study | Ro5-3335 is identified as an inhibitor of RUNX1–CBFβ function in zebrafish models. Ro5-3335 rescues preleukemic phenotype in a RUNX1-ETO transgenic zebrafish. Ro5-3335 (300 mg/kg/d; p.o; for 30 days) reduces leukemia burden in a mouse CBFB-MYH11 leukemia model. Animal Model: C57BL/6 mice (leukemic model) Dosage: 300 mg/kg Administration: Oral administration; daily; for 30 days Result: Reduced the number of c-kit + cells in the transplanted mice and leukemic cell infiltration in the livers, bone marrow and spleen. |
7-Chloro-5-(1H-pyrrol-2-yl)-1H-benzo[e][1,4]diazepin-2(3H)-one - Introduction
Ro 5-3335 is an organic compound with the molecular formula C12H7ClN4O. The following is a description of the properties, uses, preparation and safety information of the compound:
Nature:
-Appearance: Ro 5-3335 is a white or pale yellow crystalline solid.
-Melting point: About 220-222 ℃.
-Solubility: Soluble in organic solvents (such as chloroform, dichloromethane), insoluble in water.
Use:
- Ro 5-3335 is an important organic synthesis intermediate, which is widely used in the synthesis reaction of medicine, pesticide and other fields.
-It can be used to prepare biologically active compounds, such as anti-tumor drugs, antibacterial drugs, etc.
Preparation Method:
- Ro 5-3335 can be synthesized by a variety of methods, one of which is commonly used to react dichlorozhen-2 (3H)-one with 1h-pyrrole in carbon chloride, the reaction is then catalyzed with aluminum chloride.
Safety Information:
-This compound is an organochlorine compound, which has certain toxicity and irritation.
-Safety measures such as wearing protective gloves, goggles and protective clothing are required when handling and handling the compound.
-Avoid inhaling the dust or gas of the compound and avoid skin contact.
-When storing and using this compound, follow appropriate safety procedures to ensure good ventilation in the laboratory or workplace.
Nature:
-Appearance: Ro 5-3335 is a white or pale yellow crystalline solid.
-Melting point: About 220-222 ℃.
-Solubility: Soluble in organic solvents (such as chloroform, dichloromethane), insoluble in water.
Use:
- Ro 5-3335 is an important organic synthesis intermediate, which is widely used in the synthesis reaction of medicine, pesticide and other fields.
-It can be used to prepare biologically active compounds, such as anti-tumor drugs, antibacterial drugs, etc.
Preparation Method:
- Ro 5-3335 can be synthesized by a variety of methods, one of which is commonly used to react dichlorozhen-2 (3H)-one with 1h-pyrrole in carbon chloride, the reaction is then catalyzed with aluminum chloride.
Safety Information:
-This compound is an organochlorine compound, which has certain toxicity and irritation.
-Safety measures such as wearing protective gloves, goggles and protective clothing are required when handling and handling the compound.
-Avoid inhaling the dust or gas of the compound and avoid skin contact.
-When storing and using this compound, follow appropriate safety procedures to ensure good ventilation in the laboratory or workplace.
Last Update：2024-04-09 01:59:53
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